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human monocytic leukemia cell line  (ATCC)


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    ATCC human monocytic leukemia cell line
    Human Monocytic Leukemia Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp+1+cells/THP-1/pmc13126472-368-12-25
    Average 99 stars, based on 20255 article reviews
    human monocytic leukemia cell line - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Targeting PD-L1 and disrupting Siglec-9 cooperatively potentiate macrophage phagocytosis of breast cancer via a trispecific macrophage engager (TriME).
    Article Snippet: MDMs were stained with antibodies specific to macrophage markers (CD45, CD14, CD16 and CD11b, BioLegend, San Diego, CA, USA) and analyzed by flow cytometry (Fortessa, BD Biosciences, San Jose, CA, USA). .. THP− 1 cells (TIB− 202), HEK293T cells (CRL− 3216), MDA-MB− 231 cells (HTB− 26), and NK92 cells were obtained from the American Type Culture Collection (ATCC, American Type Culture Collection, Manassas, VA, USA). .. THP− 1 cells were maintained in complete RPMI− 1640 medium: RPMI− 1640 supplemented with 10%FBS, 1XGlutaMAX (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin, and 100 μg/ mL streptomycin (Gibco, Grand Island, NY, USA).

    Cell Culture:

    Article Title: Small molecule inhibitors of TLR2 signaling
    Article Snippet: .. THP-1 cells (ATCC, Manassas, VA) were cultured in RPMI 1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, 1500 mg/L sodium bicarbonate and supplemented with 10% heat-inactivated FBS. ..

    Article Title: Long-chain S-acylation is a key modulator during the macrophage inflammatory response.
    Article Snippet: .. THP-1 cells (ATCC, TIB-202, obtained from the Vidarsson lab stock at Sanquin) with a passage number below 20 were cultured in “full RPMI” medium consisting of RPMI-1640 (Capricorn, RPMISTA) supplemented with 10% fetal bovine serum (FBS, Gibco, A5256701), 100 U/ml Jo urn al Pr e-p roo f 4 penicillin/streptomycin (pen/strep, Gibco, 15140-122). .. THP-1 cells were kept at concentrations between 3e5-1e6 live cells/ml in standing culture flasks (Greiner).

    Article Title: Deficiency of Inactive Rhomboid Protein 2 (iRhom2) Attenuates Macrophage Atherogenicity.
    Article Snippet: .. For monocytes, cryopreserved THP-1 cells (ATCC, TIB-202) were cultured in RPMI supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. ..

    Modification:

    Article Title: Small molecule inhibitors of TLR2 signaling
    Article Snippet: .. THP-1 cells (ATCC, Manassas, VA) were cultured in RPMI 1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, 1500 mg/L sodium bicarbonate and supplemented with 10% heat-inactivated FBS. ..

    Derivative Assay:

    Article Title: Kefir peptides attenuate intestinal injury induced by combined exposure to microplastics and particulate matter.
    Article Snippet: .. THP− 1 cells, derived from human acute monocytic leukemia, were obtained from ATCC. .. THP− 1 cells were labeled with 10 mM BCECF/AM (Thermo, MA, USA) for 1 h at 37◦C and subsequently co-cultured with C2BBe1 cells for 1 h at 37◦C.

    other:

    Article Title: Optimized cumate toolkit for tunable protein expression during in vitro and in vivo studies of Burkholderia cenocepacia .
    Article Snippet: Proteins were transferred to a nitrocellulose membrane using the iBlot 3 Western transfer system (Thermo Fisher Scientific) and blocked for 1 h in 5% skim milk in TBS-T (Tris buffered saline with 0.1% Tween-20).



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    Image Search Results


    USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions in THP-1 after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.

    Journal: iScience

    Article Title: Ultra short waves alleviate ulcerative colitis via TRPV2 mediated-macrophage polarization

    doi: 10.1016/j.isci.2026.115929

    Figure Lengend Snippet: USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions in THP-1 after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.

    Article Snippet: Human THP-1 cells , ATCC , TIB-202.

    Techniques: Fluorescence, Imaging, Software